A butterfly in the Academy rainforest exhibit bears a torn wing, revealing the inside story.
Science writers attending the American Geophysical Union’s 2009 fall meeting in San Francisco were treated to a Behind-the-Scenes tour of California Academy of Sciences, a tour that ranged from modern DNA sequencing labs to specimen collections dating back a century.
The tour guides were both members of the Academy’s research staff, W. Brian Simison, Ph.D., Curator and Head of the Center for Comparative Genomics, and Maureen Flannery, M.S., Collections Manager for Ornithology and Mammalogy.
Simison’s research includes the evolutionary history of organisms and how they are related to each other (phylogeny), determined in part by DNA sequencing; a portion of Flannery’s work is collecting marine mammal data on Bay Area beaches. The research staff includes 24 Ph.D.-level curators, as well as approximately 12 collection managers.
The Academy's albino alligator is an example of an animal with a DNA mutation.
The research labs take up both wings of two floors of the new Academy building. Our tour started in the area reserved for spider experts (arachnologists) and insect specialists (entomologists), where Simison mentioned that older spiders such as tarantulas, evolutionarily speaking, tend to be larger with vertically moving jaws, while newer spiders were generally smaller with jaws that opened and closed horizontally. Spider and insect specimens could be placed in jars with ethanol–which preserves their DNA as well as their body parts–or pinned onto boards to dry.
The Academy also studies small specimens, such as ants, by shooting digital pictures using a CCD camera attached to a microscope. The camera takes multiple photos, each focused on a slightly different portion of the ant or other specimen; these photos are stitched together with dedicated software to provide a 3-D image of the organism’s exterior.
Vials of fossil foraminifera, unicellular (having one cell) oceanic plankton, donated to the Academy.
We walked down the halls to the next set of labs, passing box after box holding thousands upon thousands of vials of fossil “forams” donated by an oil company to the Academy’s collection. Foraminifera are oceanic plankton, or tiny floating organisms, with calcium carbonate containing tests. As part of the Academy collection, these would be preserved indefinitely for future scientific work.
Next we arrived at the DNA labs, which included separate rooms for DNA extraction, DNA amplification (making multiple copies of DNA), and DNA sequencing. The separation of tasks, noted Simison, minimized sample contamination, although when prodded by one of the reporters, he admitted to occasionally sequencing his own DNA by mistake.
Dr. W. Brian Simison discusses the Freezer Room, which preserves hundreds of valuable samples.
The following tour stop was the Freezer Room, equivalent to a bank vault for tissue and DNA samples. These are so valuable that both the room itself as well as individual freezers alarm staff if their temperature rises. (The Academy has sufficient diesel generating power to keep the freezer room in business during an entire week without power, for example, after a large earthquake.) Small tissue samples or, in some cases, aliquots of DNA, are shared with researchers around the world.
Maureen Flannery stepped in at this point to give us a snapshot of the bird and mammal collection, part of the 26 million total specimens at the Academy. Not everything was neatly tucked away in drawers, however, as sizes varied from hummingbird eggs to a blue whale skeleton.
Skeleton in storage.
We walked past rows and rows of cases, with occasional mounted skeletons or positioned birds breaking the sea of metal. Identifying a favorite location, Flannery opened a drawer containing Green Towhees collected in Peru from 1950 to 1952. She pulled on gloves before handling them, just in case these older specimens had been preserved with arsenic. These were “skins,” that is birds preserved without their inner organs.
Cabinet drawer containing a series of bird skins, part of the Academy collection.
Most valuable, mentioned Flannery, are drawers containing a group or series of individual birds from the same location, collected at the same time. A series gives an indication of the how much a species varies at a single spot.
Another avian drawer contained Birds of Paradise, originally housed in the De Young art museum, specimens that had arrived, unfortunately, without collection data. Males of this group have stunning decorative feathers used in displays that attract mates. One of the birds had been dried in a tell-tale position that betrayed its former use as a hat ornament, Flannery mentioned. Rather than use as fashion statements, however, most Academy specimens serve ecological purposes, for example, providing clues for researchers sleuthing the entry of avian pox virus into the Galapagos Islands.
Leaving the birds and moving on to the Academy's impressive collection of marine mammal skulls, Flannery retrieved a sea otter skull from one of hundreds of boxes and pointed out an imbedded shark tooth. It was confirming evidence that the otter, discovered with a missing hunk of flesh, had indeed died from a shark bite...just another fascinating detail of the Academy's collection, preserved for future generations to study.–
Anne M. Rosenthal
http://www.calacademy.org/